IN-VITRO CULTURE OF PREIMPLANTATION MOUSE EMBRYOS AND DAY-12 LIMB-BUDS - EFFECTS OF SERUM AND ALBUMIN

Citation
B. Tornesi et al., IN-VITRO CULTURE OF PREIMPLANTATION MOUSE EMBRYOS AND DAY-12 LIMB-BUDS - EFFECTS OF SERUM AND ALBUMIN, Reproductive toxicology, 7(6), 1993, pp. 623-630
Citations number
34
Categorie Soggetti
Reproductive Biology",Toxicology
Journal title
ISSN journal
08906238
Volume
7
Issue
6
Year of publication
1993
Pages
623 - 630
Database
ISI
SICI code
0890-6238(1993)7:6<623:ICOPME>2.0.ZU;2-2
Abstract
The effects of three different protein sources at different concentrat ions on the growth and development of preimplantation mouse embryos; a nd day 12 mouse limb-buds in culture were studied. Mouse embryos and f orelimb-buds were cultured with a range of concentrations (5.5 to 42%) of either donor bovine serum (DBS) or fetal bovine serum (FBS), or (0 .2 to 0.8%) bovine serum albumin (BSA). After 48 h in culture, the rat e of embryo development was significantly higher in 5.5% DBS than in a ll other groups (P < 0.05). The embryo hatching rate was higher in 21% FBS, 42% FBS, and all DBS groups than in serum-free medium, and all B SA groups (P < 0.05). Morphologic analysis of cultured limb-buds at 72 h revealed that total, paw, and cartilage area were greater (P < 0.05 ) in the serum-free medium than in all other groups. Shape factor anal ysis suggested that 5.5% DBS was most beneficial,to mouse limb-bud dev elopment. No differences were seen in DNA or protein content of limb-b uds among groups. Results suggest that mouse forelimb-buds can be succ esfully cultured in serum-free medium and that high concentrations of FBS and DBS may be detrimental for preimplantation embryo and/or limb- bud growth and development.