P. Jolivet et al., OXIDATION OF S-35 LABELED ELEMENTAL SULFUR BY WHEAT CHLOROPLASTS AND ANALYSIS OF S-35 PRODUCTS, Phytochemistry, 34(6), 1993, pp. 1467-1471
S-35(0) was offered to wheat intact chloroplasts and S-35 products ana
lysed. Radioactivity in SO42- was determined after precipitation by Ba
Cl2. Bimane derivatives of S compounds were separated by HPLC and dete
cted by fluorimetry and radioactivity monitoring. It has been observed
that fresh chloroplasts were able to oxidize S-35(0) to (SO42-)-S-35
via the production of (SO42-)-S-35 and (SO32-)-S-35. From the characte
ristics of this oxidation, the operation of chloroplastic enzymatic me
chanisms is proposed.