DETERMINATION OF DIETHYLSTILBESTROL GLUCURONIDE IN BEEF BY GAS-CHROMATOGRAPHY

A gas-liquid chromatographic (GLC) method was developed for the determ ination of diethylstilbestrol glucuronide (DESG), a metabolite of diet hylstilbestrol, in beef. DESG was extracted from beef with 90% acetoni trile. The extract was cleaned up without hydrolysis of DESG by Sep-pa k NH2 cartridge and C-18 cartridge. The purified extract was evaporate d to dryness and reacted with pentafluoropropionic acid anhydride (PFP A) for 50 min at 50 degrees C. PFPA derivatives of DESG were measured by GLC, using a 2% OV-17 column and electron capture detector. Recover ies of DESG obtained from beef spiked at 0.005-0.02 mu g/g (as is DES) levels were 86.8-89.2%. The detection limit of DESG was O.OO1 mu g/g.