AN INTESTINAL XENOGRAFT MODEL FOR CRYPTOSPORIDIUM-PARVUM INFECTION

Paired segments of near-term fetal rabbit small intestine were transpl anted subcutaneously into athymic nude mice. At 5 weeks postsurgery, t he xenografts were inoculated intraluminally with Cryptosporidium parv um sporozoites. Parasites rapidly and reliably infected the xenograft mucosal epithelium. Lesions typical of cryptosporidiosis were readily apparent by light microscopy and scanning and transmission electron mi croscopy. Xenografts are well suited to the study of the early events of C. parvum infection and are of potential value in the evaluation of anticryptosporidial chemotherapeutic agents.