Ag. Johansson et T. Skogh, DIFFERENT ELIMINATION OF CIRCULATING IGA IMMUNE-COMPLEXES IN RAT AND GUINEA-PIG - BLOOD CLEARANCE, ORGAN DISTRIBUTION AND CELLULAR UPTAKE IN THE LIVER, International archives of allergy and immunology, 103(1), 1994, pp. 36-43
Soluble IgA immune complexes, formed between I-125-labelled dinitrophe
nyl-conjugated human serum albumin and mouse IgA anti-dinitrophenyl an
tibodies (MOPC 315), were given intravenously to rats and guinea pigs.
Blood clearance kinetics and organ distribution of radioactivity were
measured after 15 min. Radioactivity was quantified in isolated paren
chymal cells, Kupffer cells (KCs) and liver endothelial cells. IgA com
plexing did not affect the antigen blood clearance kinetics in either
species. In rats, IgA increased the total hepatic antigen uptake, owin
g to a vast increase in the uptake by parenchymal cells. No IgA-mediat
ed effect could be shown in KCs, still about 40-50% of the hepatic upt
ake was confined to KCs. In guinea pigs there was also an IgA-mediated
increase in hepatic uptake, but here the increase could be ascribed t
o the KCs alone.