J. Allard et al., REDUCED SODIUM-PUMP ACTIVITY IN INOSITOL-DEFICIENT HL-60 CELLS - NO EVIDENCE OF CONTROL BY PROTEIN-KINASE-C, Biochimica et biophysica acta, 1220(1), 1993, pp. 66-68
HL-60 cells were cultured in normal and inositol-deficient media. The
inositol-deficient cells showed reduced sodium pump activity, as measu
red by ouabain-sensitive Rb-86+ uptake. The protein kinase C inhibitor
s staurosporine and H7 did not affect uptake in either normal or inosi
tol-deficient cells. However, U73122, a steroidal inhibitor of phospho
inositidase C, inhibited uptake in both types of cells. Activators of
protein kinase C had no effect on Rb+ entry. The inositol deficiency i
s not considered to affect the sodium pump by a mechanism involving di
acylglycerol and protein kinase C.