ANTILIPOLYTIC ACTION OF HESPERETIN IN RAT ADIPOCYTES

Hesperetin was able to inhibit both epinephrine- and theophylline-indu ced lipolysis in rat adipocytes. At concentrations of 125 muM and 250 muM, it could augment the inhibitory effect of low insulin concentrati on (17 muU/2ml) on epinephrine-induced lipolysis. The basal cyclic AMP levels and phosphodiesterase activities in intact adipocytes were una ltered by hesperetin. The antilipolytic action of hesperetin in adenos ine deaminase pretreated adipocytes was abolished. Hesperetin could on ly prevent lipolysis but not reverse the initial lipolytic activity st imulated through the adenosine deaminase pretreatment of the adipocyte s. In pertussis or cholera toxin (PT or CT) pretreated adipocytes, the antilipolytic action of hesperetin was unaffected. From the data obta ined it is possible to conclude that the antilipolytic action of hespe retin is not mediated through the inhibition of cyclic AMP level nor t hrough CT- or PT-sensitive G proteins. However, the involvement of hes peretin in preventing the accessibility of cellular receptors to the a ctions of lipolytic agents may be implicated.