The antibacterial and antifungal properties of the methanol, citric ac
id, aqueous, dichloromethane and petroleum ether extracts from the bar
k of Dalbergia melanoxylon were determined by using seeded agar plates
with wells into which were placed the extract, and flasks of yeast ex
tract and sucrose broth for mycelial growth of the fungi. After incuba
tion for 24 h, the diameter of the inhibition zone was measured for th
e antibacterial tests and after 7 days, the dry weight of the mycelia
was measured and a percentage of inhibition calculated using controls
where no extracts were added. The results obtained showed that the cit
ric acid extract exhibited strongest antimicrobial activity. The ethan
ol fraction showed significant antibacterial activity but was not sign
ificantly active against fungi. The dichloromethane extract exhibited
no activity against bacteria but showed notable activity against fungi
. The petroleum ether fraction showed no antimicrobial activity.