MAGNETIC FIELD-SENSITIVE STAGES OF ELECTR ON-TRANSFER IN ISOLATED REACTION CENTERS OF THE HIGHER-PLANT PHOTOSYSTEM-II

Citation
Rm. Sevdimaliev et al., MAGNETIC FIELD-SENSITIVE STAGES OF ELECTR ON-TRANSFER IN ISOLATED REACTION CENTERS OF THE HIGHER-PLANT PHOTOSYSTEM-II, Biofizika, 38(6), 1993, pp. 952-957
Citations number
22
Categorie Soggetti
Biophysics
Journal title
ISSN journal
00063029
Volume
38
Issue
6
Year of publication
1993
Pages
952 - 957
Database
ISI
SICI code
0006-3029(1993)38:6<952:MFSOEO>2.0.ZU;2-8
Abstract
The mechanisms of the magnetic field effect on the fluorescence yield of the chlorophyll, DELTAF(B)/F, and the optical absorbance-difference T-S spectra of the primary electron donor are studied in D1/D2/cytoch rome b-559 photosystem II complexes (isolated PS II RC) in the tempera ture range 9-295 K. It is demonstrated that in the temperature range 4 0-295 K DELTAF(B)/F is due to superposition of two magnetic field-sens itive fluorescence components which are related to charge recombinatio n of RP (P680+I-). Occurence of these magnetic field-sensitive fluores cence components is explained by the formation of relaxed and nonrelax ed states of RP (P680+I-) and/or heterogeneity of RCs preparation. It is proposed that effect of the magnetic field on fluorescence yield in the temperature range 9-35 K is due to mixing of the eigenfunctions ( mixing of the decay rate constants and population probabilities) of th e triplet sublevels of P680 and charge recombination of RP (P680+I-) t o the ground state of P680. The investigation of absorbance-difference T-S spectra of P680 indicates that it is a dimer of chlorophyll molec ules with sufficient interactions between the two monomers in the sing let state and minor interactions in the triplet state. It is suggested that absorbance-difference T-S spectra of P680 reflect the heterogene ity of the RCs. Energy transfer from P680 triplet state to beta-carote ne in the studied D1/D2/cytochrome b-559 complexes is not observed.