APPLICATION OF FE2-INDUCED CHEMILUMINESC ENCE KINETICS IN TRIS-BUFFERLIPOSOME SUSPENSION FOR DETERMINATION OF BLOOD-PLASMA ANTIOXIDANT ACTIVITY()

In order to evaluate antioxidant activity (AOA) of different preparati ons in respect to Fe2+-induced lipid peroxidation (LPO) chemiluminesce nce (CL) kinetics in tris-buffer liposome suspension was proposed. For studying AOA stages of fast flash, latent period and slow flash relat ed to LPO reaction development were used. The measurement of CL kineti c parameters along with malondialdehyde accumulation makes it possible to determine also the mechanism of antioxidant effect on the preparat ions under consideration. The mechanism of AOA of the blood plasma and its components were investigated. It was found that AOA of the blood plasma is mainly due to transferrin, ceruloplasmin and HCO3- interacti on with Fe2+ leading to abstraction of some part of ferrous ions parti cipated in LPO reactions. In the case of albumin the change of CL kine tic parameters was proposed to be conditioned by the competition with ferrous ions for binding sites on liposomes. The effect of alpha-tocop herol at physiological concentrations compared with other blood plasma components in this system was negligibly small.