DNA PROBES FOR ALTERNATIVE SPLICING REGIO N OF THE 6-EXON HUMAN CSF-1GENE - POLYMERASE CHAIN-REACTION AND SUBCLONING

The DNA probes - pA6-CSF-1 and pA2-CSF-1 for the alternative splicing region of the 6 exon human CSF-1 gene were prepared using PCR and subs equent subcloning in pUC19 plasmid at the XmaI/BamHI sites. Due to the insert sequencing and blotting of human leukocytes DNA, the DNA probe s obtained can be useful for screening of mutations in the human CSF-1 gene.