REAL-TIME OBSERVATION OF LOW-FREQUENCY HEME PROTEIN VIBRATIONS USING FEMTOSECOND COHERENCE SPECTROSCOPY

Authors
ZHU L LI P HUANG M SAGE JT CHAMPION PM
Citation
L. Zhu et al., REAL-TIME OBSERVATION OF LOW-FREQUENCY HEME PROTEIN VIBRATIONS USING FEMTOSECOND COHERENCE SPECTROSCOPY, Physical review letters, 72(2), 1994, pp. 301-304
Citations number
30
Categorie Soggetti
Physics
Journal title
Physical review letters → ACNP
ISSN journal
00319007
Volume
72
Issue
2
Year of publication
1994
Pages
301 - 304
Database
ISI
SICI code
0031-9007(1994)72:2<301:ROOLHP>2.0.ZU;2-2
Abstract
Femtosecond laser pulses, resonant with the Soret bands of myogiobin ( Mb) and cytochrome c, are used to probe coherent low frequency nuclear motion of the heme group. The time domain analysis is in good agreeme nt with frequencies obtained independently using spontaneous resonance Raman spectroscopy. The deoxyMb data reveal a strong oscillation near 300 fs (approximately 100 cm-1) and a persistent feature also appears near 50 cm-1. This is near the frequency expected for heme doming mot ion, which has been associated with the ligand binding reaction coordi nate of Mb.