SEPARATION OF OXYTETRACYCLINE AND ITS RELATED SUBSTANCES BY CAPILLARYELECTROPHORESIS

A method is described for separation of oxytetracycline and its relate d substances by capillary electrophoresis. Oxytetracycline is a broad spectrum antibiotic obtained by biosynthesis. Main related substances are 4-epioxytetracycline, alpha-apooxytetracycline, beta-apooxytetracy cline, anhydrooxytetracycline, 2-acetyl-2-decarboxamidooxytetracycline tetracycline, 4-epitetracycline and terrinolide. Method development w as undertaken with a mixture consisting of oxytetracycline and its rel ated substances mentioned. Using an uncoated fused silica capillary, t he influence of buffer pH and its concentration was systematically inv estigated. Non-ionic surfactant Triton X-100 was added to the running buffer for improving separation selectivity. Quantitative data are rep orted and compared with those of liquid chromatography and capillary z one electrophoresis.