IMMUNOHISTOCHEMICAL OBSERVATIONS OF MACROPHAGES AND PERISINUSOIDAL CELLS IN CARBON TETRACHLORIDE-INDUCED RAT-LIVER INJURY

In rat liver injured by carbon tetrachloride, ED-1-positive monocytes and resident macrophages significantly increased in number in damaged perivenular zones on days 2 and 4 after single or double dosing, peaki ng on day 2 and being two-fold more after the 2nd dosing. ED-2-positiv e resident macrophages significantly increased in number only on day 4 after the single dosing and on days 2, 4 and 6 after the 2nd dosing w ith a peak on day 2. A significant increase in number of muscle actin- positive perisinusoidal cells was seen on day 4 and on days 2 and 4 af ter the Ist and 2nd dosings, respectively, reaching a peak on day 4 an d being more remarkable after the 2nd dosing.