USE OF B95A CELLS FOR ISOLATION OF CANINE-DISTEMPER VIRUS FROM CLINICAL CASES

Canine distemper virus (CDV) was readily isolated at high rate with ma rked cytopathic effect (CPE) in B95a cells, a marmoset lymphoid cell l ine, from the peripheral blood leukocytes, cerebrospinal fluid cells a nd brain of dogs. Difference in type of CPE, i.e. syncytium type and r ound-cell one, among the virus isolates indicate the presence of heter ogeneity of virus populations in prevalent CDV. Thus, this cell system is expected to be useful for ecological studies on CDV in the field.