CHARACTERIZATION AND APPLICATION OF UV DETECTOR SPORE FILMS - THE SENSITIVITY CURVE OF A NEW DETECTOR SYSTEM PROVIDES GOOD SIMILARITY TO THE ACTION SPECTRUM FOR UV-INDUCED ERYTHEMA IN HUMAN SKIN
Le. Quintern et al., CHARACTERIZATION AND APPLICATION OF UV DETECTOR SPORE FILMS - THE SENSITIVITY CURVE OF A NEW DETECTOR SYSTEM PROVIDES GOOD SIMILARITY TO THE ACTION SPECTRUM FOR UV-INDUCED ERYTHEMA IN HUMAN SKIN, Journal of photochemistry and photobiology.B, Biology, 37(1-2), 1997, pp. 158-166
The UV action spectra of two different biologically weighing UV photof
ilms (spore films), produced with Bacillus subtilis spores (wildtype a
nd DNA repair-deficient strains), were determined at the Okasaki large
spectrograph (OLS) within the wavelength range 254-400 nm. The action
spectrum of the mutant strain film was modified with a cut-off filter
, yielding a sensitivity curve similar to the action spectrum for eryt
hemal induction in human skin. The detector system was tested in a fie
ld study and in a study using lamps with different UV spectral composi
tions. The system demonstrated its applicability over the spectral reg
ion from lambda=290 nm to the visible light, The system could be calib
rated to give the minimal erythemal dose.