IN-VITRO FERTILIZATION USING JAPANESE MON KEY (MACACA-FUSCATA) EGGS AND CYNOMOLGUS MONKEY (MACACA-FASCICULARIS) FROZEN THAWED SPERMATOZOA/

Using four female Japanese monkeys, follicular growth was induced by m ultiple PMSG and single hCG injections, and oocytes were collected fro m their follicles. PMSG, 200 IU, was injected six or eight times to st imulate follicular growth, and in both cases growth of the follicles w as adequately induced. Forty-seven (50.0%) oocytes were recovered from 94 follicles of two of the females and transported from Inuyama-shi t o Tsukuba-shi in 10 hours. Five mature oocytes and 42 immature oocytes were observed upon arrival. Thirteen (31.0%) of the 42 immature oocyt es released the first polar body in TCM-199 medium containing FCS and PMSG after 24 hour-culture, including transport time. Eighteen mature oocytes were used for in vitro fertilization (IVF). Frozen and thawed cynomolgus monkey spermatozoa preincubated for 2 hours were transferre d into the drops containing the Japanese monkey oocytes. Sixteen oocyt es (88.9%) were judged to have been success fully fertilized based on the presence of a male pronucleus and/or second polar body, and 12 ooc ytes then cleaved, and 3 developed into the 6-cell stage. Thus, we suc ceeded in inducing follicular growth and oocyte maturation, and in ach ieving IVF using cynomolgus monkey spermatozoa and Japanese monkey ooc ytes.