DETECTION OF CONFORMATION BIAS BETWEEN THE COMPLEMENTARY STRANDS OF DEOXYNUCLEOTIDE REPEAT (TG AC)(N)/

Antiparallel complementary strands of DNA contribute symmetrically to the helical structure and its stability; rough conformational differen ces between complementary strands would interfere with this symmetric contribution and the stability. To detect conformational differences b etween complementary strands, we challenged the complementary strands to different ''pore size'' of acrylamide gels and measured differences in migration during electrophoretic mobility analysis. Changes in mig ration differences revealed a significant structural bias between the complementary strands of the dinucleotide repeat deoxy-(TG/AC)(n).