NEW CATALYTIC PROPERTIES OF GLUCOSE-OXIDASE IN MONOMOLECULAR FILMS

Glucose oxidase in monomolecular films obtained from Penicillium vital e based on polymeric matrices were studied with a Clark-type oxygen el ectrode. The kinetic constants of the immobilized and dissolved forms of the enzyme were compared. The kinetic activity of glucose oxidase i n monomolecular films was assayed under various conditions of applicat ion, measurement and storage. The data obtained indicate that glucose oxidase immobilized on positively charged surfaces (polyethyleneimine) allow the construction of a glucose electrode with a linear response in the range of 1-10 mM and a response time of 20 s. The formation of monomolecular glucose oxidase films results in a significant decrease of the Michaelis constant. The glucose electrode is capable of maintai ning the initial enzymic activity over 2 months.