IMMOBILIZATION AND STABILIZATION OF INVERTASE USING SPECIFIC POLYCLONAL ANTIBODIES

Antisera raised in rabbits to baker's-yeast invertase significantly ac tivated the enzyme in vitro. The antisera contained precipitating anti bodies, a significant fraction of which appeared to be directed agains t the glycosyl residues of the enzyme. Invertase could be immobilized as insoluble enzyme antibody adducts or by binding to a Sepharose matr ix precoupled with the gamma-globulin fraction derived from the antise ra. The immobilized invertase preparations exhibited high enzyme activ ity and had markedly enhanced thermal stability, which could be furthe r improved by cross-linking with glutaraldehyde.