LEUCINE SYNTHESIS IN SPINACH-CHLOROPLASTS - PARTIAL CHARACTERIZATION OF 2-ISOPROPYLMALATE SYNTHASE

Authors
HAGELSTEIN P SCHULTZ G
Citation
P. Hagelstein et G. Schultz, LEUCINE SYNTHESIS IN SPINACH-CHLOROPLASTS - PARTIAL CHARACTERIZATION OF 2-ISOPROPYLMALATE SYNTHASE, Biological chemistry Hoppe-Seyler, 374(12), 1993, pp. 1105-1108
Citations number
20
Categorie Soggetti
Biology
Journal title
Biological chemistry Hoppe-Seyler → ACNP
ISSN journal
01773593
Volume
374
Issue
12
Year of publication
1993
Pages
1105 - 1108
Database
ISI
SICI code
0177-3593(1993)374:12<1105:LSIS-P>2.0.ZU;2-3
Abstract
Leucine synthesis was found to be localized in chloroplasts of higher plants. The introductory enzyme of the isopropylmalate pathway, the 2- isopropylmalate synthase, was strongly feedback-regulated by leucine. The pH optimum of the enzyme was between 7.0 and 9.0. The K-m values w ere 5 mu M for acetyl-CoA and 75 mu M for 2-oxoisovalerate.