Re. Collis et al., THE EFFECT OF HYDROXYETHYL STARCH AND OTHER PLASMA-VOLUME SUBSTITUTESON ENDOTHELIAL-CELL ACTIVATION - AN IN-VITRO STUDY, Intensive care medicine, 20(1), 1994, pp. 37-41
Objective: To study the effect of medium molecular weight hydroxyethyl
starches on endothelial cell and neutrophil activation in vitro. Sett
ing: Laboratory analysis. Methods: The effects of albumin and hydroxye
thyl starch on the neutrophil adhesion molecule (CD11bCD18), with and
without liposaccharide stimulation, were studied in whole blood. E-sel
ectin expression on human umbilical vein endothelial cells was stimula
ted with lipopolysaccharide alone and in the presence of either albumi
n or hydroxyethyl starch. The effect of albumin and hydroxyethyl starc
hes on rapid endothelial cell activation was studied using von Willebr
and factor release as a marker. Measurements and results: Hydroxyethyl
starches but not albumin inhibited stimulated VWF release in a dose d
ependent manner. No effect was seen on endothelial E-selectin or neutr
ophil CD11bCD18 expression. Conclusions: these results suggest a possi
ble beneficial role of hydroxyethyl starches in the inhibition of endo
thelial activation thus preventing neutrophil adhesion during sepsis s
yndrome.