Jc. Haigh et al., COMPARISON OF 2 EXTENDERS FOR THE CRYOPRESERVATION OF CHITAL (AXIS-AXIS) SEMEN, Journal of zoo and wildlife medicine, 24(4), 1993, pp. 454-458
Electroejaculates were collected from chital deer (Axis axis) and froz
en in pellets (0.1 ml/pellet) on dry ice after extension with either T
riladyl(TM) or a Tris-egg-yolk-citrate extender. The semen was thawed
in a 36 degrees C water bath for 1 min and evaluated for percentage sp
erm motility and rate of forward progression immediately after thawing
and at 2 hr. The Triladyl-extended semen had significantly greater mo
tility immediately after thawing, but after incubation the Tris-egg-yo
lk-citrate-extended semen showed a significantly higher proportion of
motile sperm.