ENDOTHELIAL-CELL SEEDING KINETICS UNDER CHRONIC FLOW IN PROSTHETIC GRAFTS

Improved patency of endothelial cell seeded grafts relies on good init ial adherence and cell retention when the circulation is restored. In this study human adult endothelial cells (HAECs) were used to evaluate the suitability of commercially available prostheses for seeding. Acu tely seeded indium-111 oxine labeled HAECs were used to measure cell a dherence to plain and fibronectin (FN)-coated expanded polytetrafluoro ethylene (ePTFE), gelatin-impregnated Dacron (Gelseal), and collagen-i mpregnated Dacron (Hemashield) grafts. Cell loss from FN-coated prosth eses, when exposed to a simulated human arterial blood flow of 200 ml/ min in an artificial pulsatile circulation, was quantified from the lo ss of gamma activity from the graft over 24 hours, pressure in the cir culation being reduced to 15 mm Hg to reduce fluid loss. Initial HAEC adherence (mean [SD]) to plain grafts was 3(1)%, 47(9)%, and 53(9)% fo r ePTFE, Gelseal, and Hemashield, respectively. This improved signific antly with FN coating (78[6]%, 60[8]%, and 76[4]%). Cell retention aft er 24 hours of flow to FN-coated grafts was 16(10)%, 25(5)%, and 65(4) % and was confirmed qualitatively by scanning electron microscopy and environmental scanning electron microscopy. FN significantly improved initial cell adherence with Dacron grafts showing the better adherence . Cell retention after 24 hours of flow was better with FN-coated Dacr on than with ePTFE but was best with Hemashield grafts.