BIOCONCENTRATION AND METABOLISM OF 3,4-DICHLOROANILINE IN DIFFERENT LIFE STAGES OF GUPPY AND ZEBRAFISH

Bioconcentration factors (BCF) and metabolism of 3,4-dichloroaniline i n different life stages of guppy (Poecilia reticulata Peters) and zebr afish (Brachydanio rerio Hamilton-Buchanan) were investigated. The res ults prove that embryos of zebrafish, four-day-old yolk sac larvae, 17 -day-old larvae and adult zebrafish are able to transform the chemical to 3,4-dichloroacetanilide. Bioconcentration factors in these life st ages are between 30 and 42, exept yolk sac larvae showing a BCF of 86. 3,4-Dichloroacetanilide was also detected in extracts of newborn and adult guppies, but they are able to form an additional, yet unknown me tabolite. The BCF varies from 34 in six-month-old guppies to 226 in ne wborns. Tissue distribution of 3,4-dichloroaniline and its metabolites in adult guppies demonstrates high concentrations in gallbladder and intestine. Metabolite pattern of embryos (one week before hatching) ar e nearly identical to those of adult female guppies. In zebrafish ther e are in any tissues or organs nearly same concentrations of the chemi cal.