SCHISTOSOMA-MANSONI - DETERMINATION OF THE SEX OF CERCARIAE USING A NONRADIOACTIVELY LABELED DNA-PROBE

Authors
GIBODA M CAMPOS EG SMITH JM PRICHARD RK
Citation
M. Giboda et al., SCHISTOSOMA-MANSONI - DETERMINATION OF THE SEX OF CERCARIAE USING A NONRADIOACTIVELY LABELED DNA-PROBE, Tropical medicine and parasitology, 44(4), 1993, pp. 335-336
Citations number
3
Categorie Soggetti
Parasitiology,"Tropical Medicine
Journal title
Tropical medicine and parasitology → ACNP
ISSN journal
01772392
Volume
44
Issue
4
Year of publication
1993
Pages
335 - 336
Database
ISI
SICI code
0177-2392(1993)44:4<335:S-DOTS>2.0.ZU;2-N
Abstract
Cercarial sex determination protocol which utilizes nonradioactive nuc leic acid labelling with digoxigenin (DIG)-11-dUTR, offers a rapid ''c lean'' identification of the sex of Schistosoma mansoni cercaria. DIG labelled pW1-DNA (plasmid female-specific genomic DNA clone - W1) hybr idized with the native cercarial DNA, and hybridization signals are vi sualized with Lumigen PPD. Results obtained are comparable with those obtained with the P-32 labelled pW1.