PURIFICATION AND PARTIAL SEQUENCE OF PROTEINS INVOLVED IN THE CHOLIC-ACID TRANSPORT INTO RAT-LIVER HEPATOCYTES

Authors
HOFFMANN A MULLNER S ZIEGLER K FASOLD H
Citation
A. Hoffmann et al., PURIFICATION AND PARTIAL SEQUENCE OF PROTEINS INVOLVED IN THE CHOLIC-ACID TRANSPORT INTO RAT-LIVER HEPATOCYTES, Journal of protein chemistry, 12(6), 1993, pp. 765-769
Citations number
18
Categorie Soggetti
Biology
Journal title
Journal of protein chemistry → ACNP
ISSN journal
02778033
Volume
12
Issue
6
Year of publication
1993
Pages
765 - 769
Database
ISI
SICI code
0277-8033(1993)12:6<765:PAPSOP>2.0.ZU;2-F
Abstract
Two proteins, in previous work labeled by affinity markers derived fro m taurocholic acid, were purified and partially sequenced. Antibodies were raised against purified proteins, and cross-reactions were carefu lly checked. The influence of these antibodies upon taurocholic acid i mport into vesicles from rat liver plasma membranes was measured, and showed a distinct inhibition of transport in the case of the 54 kD pro tein.