Chromosome studies of 11 male and four female sloths of the species Br
adypus variegatus were performed. These animals showed two types of ka
ryotypes: most (13 animals) presented 2n=54 chromosomes and two mares
and 2n=55 chromosomes, with a supposed additional Y chromosome. The pa
ttern of constitutive heterochromatin in this species showed a great s
ize variation.