Ms. Mantovani et al., EVALUATION OF THE GENOTOXIC ACTIVITY OF THE SESQUITERPENE LACTONE GOYAZENSOLIDE IN MAMMALIAN SYSTEMS IN-VITRO AMD IN-VIVO, Brazilian journal of genetics, 16(4), 1993, pp. 967-975
Tile sesquiterpene lactone goyazensolide)ras schistosomicidal and cyto
toxic activities. This chemical substance was tested to determine clas
togenic effects on mammalian systems in vitro (human peripheral blood
lymphocyte cultures) and in vivo (Wistar rat bone marrow cells). Tile
concentrations tested in vitro were 0.1, 0.3, 0.6 and 1.0 mu g/ml cult
ure medium, and in vivo were 0.4, 0.8, 1.6 and 3.2 mg/100 g body weigh
t. All in vitro treatments with goyazensolide and only die 0.8 mg in v
ivo treatment had significantly more chromosome gaps than the controls
. However, only in vitro analysis of the number of cells with chromoso
me breaks demonstrated that treatment with 0.6 pg goyazensolide/ml cul
ture medium has a clastogenic action. Goyazensolide was found to have
no effect on SCE induction in vitro. Tile toxic dose was determined bo
th in vitro (1.0 mu g/ml) and in vivo (3.2 mg/100 g).