EVALUATION OF THE GENOTOXIC ACTIVITY OF THE SESQUITERPENE LACTONE GOYAZENSOLIDE IN MAMMALIAN SYSTEMS IN-VITRO AMD IN-VIVO

Tile sesquiterpene lactone goyazensolide)ras schistosomicidal and cyto toxic activities. This chemical substance was tested to determine clas togenic effects on mammalian systems in vitro (human peripheral blood lymphocyte cultures) and in vivo (Wistar rat bone marrow cells). Tile concentrations tested in vitro were 0.1, 0.3, 0.6 and 1.0 mu g/ml cult ure medium, and in vivo were 0.4, 0.8, 1.6 and 3.2 mg/100 g body weigh t. All in vitro treatments with goyazensolide and only die 0.8 mg in v ivo treatment had significantly more chromosome gaps than the controls . However, only in vitro analysis of the number of cells with chromoso me breaks demonstrated that treatment with 0.6 pg goyazensolide/ml cul ture medium has a clastogenic action. Goyazensolide was found to have no effect on SCE induction in vitro. Tile toxic dose was determined bo th in vitro (1.0 mu g/ml) and in vivo (3.2 mg/100 g).