Screening cDNA libraries with polyclonal antibody preparations against
Candida albicans yeast or mycelial cell walls resulted in isolation o
f several positive clones. Some of them encoded enolase; others encode
d a protein of the 70 kDa heat-shock protein family (Hsp7Op), etc. The
presence of these cytosolic proteins in the cell wall of actively gro
wing C. albicans was discovered by analytical (SDS-PACE and Western bl
ot) and cytological (indirect immunofluorescence) experiments. Supplem
entation of cell cultures with papulacandin B, an antibiotic that inhi
bits formation of the beta-glucan skeleton, resulted in the release of
enolase to the supernatant fluids; this release was prevented when 0.
6 M KCl was present as an osmotic stabilizer. The cell wall of C. albi
cans incorporated exogenously added proteins (enolase and Escherichia
coil and C. albicans cytosolic proteins). The presence in the C. albic
ans cell wall of enolase, Hsp7Op, and probably other intracellular pro
teins that are highly immunogenic might help the fungal cells to evade
the host defences, and consequently could represent a survival mechan
ism for C. albicans 'in vivo'.