L-PHENYLALANINE PRODUCTION BY DOUBLE-AUXOTROPHIC MUTANTS OF ARTHROBACTER-GLOBIFORMIS

Authors
MAITI TK CHATTERJEE SP
Citation
Tk. Maiti et Sp. Chatterjee, L-PHENYLALANINE PRODUCTION BY DOUBLE-AUXOTROPHIC MUTANTS OF ARTHROBACTER-GLOBIFORMIS, Research and Industry, 38(4), 1993, pp. 234-237
Citations number
NO
Categorie Soggetti
Engineering, Industrial
Journal title
Research and Industry → ACNP
ISSN journal
0034513X
Volume
38
Issue
4
Year of publication
1993
Pages
234 - 237
Database
ISI
SICI code
0034-513X(1993)38:4<234:LPBDMO>2.0.ZU;2-3
Abstract
A number of tryptophan plus tyrosine double auxotrophic mutants isolat ed by NTG treatment of a glutamic acid producing strain of Arthrobacte r globiformis were found to excrete phenylalanine in mineral salt medi um. For the three potent mutants (TT-37, TT-39 and TT-55) tested the m edium of Alfoldi, glucose 350 mM, NH4Cl 60 mM and biotin 5 mug/l were the best for phenylalanine production. On the optimum levels strain TT -39 yielded 2.6 g phenylalanine per liter of medium in flask culture.