DETECTION OF A PROTEIN AND DIFFERENCES IN THE POPULATIONS OF TRANSLATABLE MESSENGER-RNA SPECIFIC TO DWARF RICE (ORYZA-SATIVA L)

To isolate genes related to dwarfism in rice, we analysed the differen ces in the expressions of cellular proteins and translation products o f mRNA between normal and dwarf rice of isogenic lines. By analysis of soluble proteins, one protein of 93 kD was detected exclusively in dw arf rices (Akibare-waisei and Hosetsu-waisei). This protein was detect ed up 2 weeks after imbibition, however it disappeared after 4 weeks. From analysis of in vitro translation products of mRNA by two-dimensio nal gel electrophoresis, thirty one differences of spots expressed by translatable mRNA were observed among normal rice, Akibare and extreme dwarf rices, Akibare-waisei and Hosetsu-waisei at 2 and 4 weeks after imbibition. In these differences, twenty spots were detected in norma l, Akibare and five spots were detected in extreme dwarf rices both Ak ibare-waisei and Hosetsu-waisei at 2 weeks. Most of the spots specific to normal and dwarf rices almost disappeared at 4 weeks, but two new spots appeared in both normal and dwarf rices.