A NEW MONOCLONAL-ANTIBODY AGAINST THE ANIONIC DOMAIN OF THE AMYLOID PRECURSOR PROTEIN OF ALZHEIMERS-DISEASE

A NEW mouse monoclonal antibody was raised to a bacterial fusion prote in between beta-galactosidase and the extracellular domain of the huma n amyloid protein precursor (APP). In immunoblotting experiments, this monoclonal antibody labelled the bacterial fusion protein used as an immunogen, the human brain APP, and different full-length APP isoforms expressed by transfected cells. In immunocytochemistry, the monoclona l antibody stained the dystrophic neurites of abundant senile plaques found in the cerebral cortex of patients with Alzheimer's disease. Usi ng bacterial expression of several cDNA fragments, the epitope was map ped to an amino acid sequence of APP not investigated before.