DETERMINATION OF NAFCILLIN AND METHICILLIN BY DIFFERENT SPECTROFLUOROMETRIC TECHNIQUES

In order to explore the possibilities of combining synchronous fluores cence and derivative spectrometry and to establish a methodology for t his type of technique, nafcillin and methicillin were determined using these techniques. Several methods for resolving binary mixtures of th ese penicillins using first derivative spectrofluorimetry, first deriv ative constant wavelength synchronous luminescence spectrometry and co nstant energy synchronous luminescence spectrometry are described. The analyses were performed in aqueous medium at pH 6.20 provided by the addition of phosphate buffer solution. A complete and exhaustive stati stical analysis of the experimental data was performed to demonstrate the validity of these methods, which obtained good results when applie d for determining nafcillin and methicillin synthetic and real mixture s.