HYDRODYNAMIC PROPERTIES OF NITROGENASE - THE MOFE PROTEIN FROM AZOTOBACTER-VINELANDII STUDIED BY DYNAMIC LIGHT-SCATTERING AND HYDRODYNAMIC MODELING

Authors
HELLWEG T EIMER W KRAHN E SCHNEIDER K MULLER A
Citation
T. Hellweg et al., HYDRODYNAMIC PROPERTIES OF NITROGENASE - THE MOFE PROTEIN FROM AZOTOBACTER-VINELANDII STUDIED BY DYNAMIC LIGHT-SCATTERING AND HYDRODYNAMIC MODELING, Biochimica et biophysica acta. Protein structure and molecular enzymology, 1337(2), 1997, pp. 311-318
Citations number
29
Categorie Soggetti
Biology,Biophysics
Journal title
Biochimica et biophysica acta. Protein structure and molecular enzymology → ACNP
ISSN journal
01674838
Volume
1337
Issue
2
Year of publication
1997
Pages
311 - 318
Database
ISI
SICI code
0167-4838(1997)1337:2<311:HPON-T>2.0.ZU;2-8
Abstract
Experimental results for the nitrogenase MoFe protein from Azotobacter vinelandii obtained by dynamic light scattering (DLS) are presented. The translational diffusion coefficient was determined to D = (4.0 +/- 0.2) x 10(-7) cm(2)/s. Complementary, we have performed hydrodynamic model calculations based on the X-ray crystallographic data of the MoF e protein. The calculated transport coefficient suggests that the size and shape of the protein in solution is consistent with that in the c rystal structure.