CYTOMETRIC VALIDATION OF IMMUNOCYTOCHEMICAL OBSERVATIONS IN DEVELOPING LUNG-CANCER

Video-enhanced optical microscopy objectively confirms that immunocyto chemical biomarker supplementation of routine cytology permits separat ion of (1) ''pre'' cancer epithelial cells from JHLP screened individu als who subsequently developed lung cancer from (2) similarly atypical epithelial cells from JHLP participants who did not. The establishmen t of a bank of sputum cytology specimens along with a data respository of 5-yr follow-up and clinical outcome had permitted banked specimens to be immunostained and scored against a gold standard of subsequent histologic lung cancer. A highly skilled cytopathologist, interpreting immunostained screening sputum specimens and blinded to the clinical outcome, originally was able to correctly predict clinical outcome wit h 88.7% accuracy compared to this ''gold standard'' (Tockman et al, J Clin Oncol 1988;6:1685-1693). This study presents an independent confi rmation of that earlier immunocytochemical classification through feat ure extraction of digitally recorded, transmission optical microscope video images of immunostained, moderately atypical sputum cell from th e original slides. (C) 1993 Wiley-Liss, Inc.