DETECTION OF GENETIC DIVERSITY WITHIN SNAIL INTERMEDIATE HOSTS OF THEGENUS BULINUS BY USING RANDOM AMPLIFIED POLYMORPHIC DNA MARKERS (RAPDS)

Citation
J. Langand et al., DETECTION OF GENETIC DIVERSITY WITHIN SNAIL INTERMEDIATE HOSTS OF THEGENUS BULINUS BY USING RANDOM AMPLIFIED POLYMORPHIC DNA MARKERS (RAPDS), Acta Tropica, 55(4), 1993, pp. 205-215
Citations number
34
Categorie Soggetti
Biology,"Tropical Medicine",Parasitiology
Journal title
ISSN journal
0001706X
Volume
55
Issue
4
Year of publication
1993
Pages
205 - 215
Database
ISI
SICI code
0001-706X(1993)55:4<205:DOGDWS>2.0.ZU;2-R
Abstract
A polymerase chain reaction-based polymorphic assay procedure (RAPD) w as used to study the genetic diversity of intermediate host snails wit hin the genus Bulinus. Genetic differentiation was detected between tw o closely related species: Bulinus globosus and Bulinus umbilicatus. E vidence is presented demonstrating the potential of RAPD markers for d ifferentiating populations of B. forskalii from different countries (C ameroon, Equatorial Guinea and Ivory Coast) or from the same country ( Cameroon). RAPDs may be also used to identify offspring from cross- an d self-fertilized hermaphrodite bulinid snails: RAPDs provide a cost-e ffective and routine method for genetic studies of snails transmitting schistosomiasis and for the evaluation of diversity between snail pop ulations.