CLONING AND EXPRESSION OF A CDNA FOR MOUSE PROSTAGLANDIN-F RECEPTOR

A functional cDNA clone for mouse prostaglandin (PG) F receptor was is olated from a mouse cDNA library using polymerase chain reaction based on the sequence of cloned prostanoid receptors, and cross-hybridizati on screening. The mouse PGF receptor consists of 366 amino acid residu es with putative seven transmembrane domains. The sequence revealed th e highest homology to the EP1 subtype of PGE receptor and thromboxane (TX) A2 receptor. Ligand binding studies using membranes of COS cells transfected with the cDNA revealed specific [H-3]PGF2alpha binding. Th e binding was displaced with unlabeled PGs in the order of PGF2alpha = 9alpha,11beta-PGF2 > PGF1alpha > PGD2 > STA2 (a stable TXA2 agonist) > PGE2 > iloprost (a stable PGI2 agonist). PGF2alpha increased inosito l trisphosphate formation in a concentration-dependent manner in COS c ells expressing PGF receptor. RNA blot and in situ hybridization analy ses demonstrated that the PGF receptor transcripts are abundantly expr essed in luteal cells of corpus luteum and in a lesser amount in kidne y, heart, stomach, and lung.