CYSTEINE BIOSYNTHESIS IN HIGHER-PLANTS - A NEW MEMBER OF THE ARABIDOPSIS-THALIANA SERINE ACETYLTRANSFERASE SMALL GENE-FAMILY OBTAINED BY FUNCTIONAL COMPLEMENTATION OF AN ESCHERICHIA-COLI CYSTEINE AUXOTROPH

Authors
HOWARTH JR ROBERTS MA WRAY JL
Citation
Jr. Howarth et al., CYSTEINE BIOSYNTHESIS IN HIGHER-PLANTS - A NEW MEMBER OF THE ARABIDOPSIS-THALIANA SERINE ACETYLTRANSFERASE SMALL GENE-FAMILY OBTAINED BY FUNCTIONAL COMPLEMENTATION OF AN ESCHERICHIA-COLI CYSTEINE AUXOTROPH, Biochimica et biophysica acta, N. Gene structure and expression, 1350(2), 1997, pp. 123-127
Citations number
27
Categorie Soggetti
Biology,Biophysics,"Biothechnology & Applied Migrobiology
Journal title
Biochimica et biophysica acta, N. Gene structure and expression → ACNP
ISSN journal
01674781
Volume
1350
Issue
2
Year of publication
1997
Pages
123 - 127
Database
ISI
SICI code
0167-4781(1997)1350:2<123:CBIH-A>2.0.ZU;2-G
Abstract
A cDNA clone, Sat-52, encoding a novel isoform of serine acetyltransfe rase (EC 2.3.1.30) was isolated by functional complementation of an Es cherichia coli cysE mutant defective in serine acetyltransferase. The 1158 base pair clone contains a full-length open reading frame encodin g a deduced protein of 312 amino acids with an M(r) of 32.77 kDa. Nort hern analysis revealed a single transcript of ca 1.19 kb that did not increase in abundance under sulfate limitation. Genomic Southern hybri dization suggests the presence of a single copy of the Sat-52 gene.