STRUCTURAL AND FUNCTIONAL-ANALYSIS OF THE 5'-FLANKING REGION OF THE HUMAN INSULIN-LIKE GROWTH-FACTOR BINDING-PROTEIN (IGFBP)-4 GENE

Authors
QIN XZ MORALES S LEE RW BOONYARATANAKORNKIT V BAYLINK DJ MOHAN S STRONG DD
Citation
Xz. Qin et al., STRUCTURAL AND FUNCTIONAL-ANALYSIS OF THE 5'-FLANKING REGION OF THE HUMAN INSULIN-LIKE GROWTH-FACTOR BINDING-PROTEIN (IGFBP)-4 GENE, Biochimica et biophysica acta, N. Gene structure and expression, 1350(2), 1997, pp. 136-140
Citations number
23
Categorie Soggetti
Biology,Biophysics,"Biothechnology & Applied Migrobiology
Journal title
Biochimica et biophysica acta, N. Gene structure and expression → ACNP
ISSN journal
01674781
Volume
1350
Issue
2
Year of publication
1997
Pages
136 - 140
Database
ISI
SICI code
0167-4781(1997)1350:2<136:SAFOT5>2.0.ZU;2-1
Abstract
More than 3 kb of the human (h)IGFBP-4 gene 5'-flanking region was seq uenced and assessed for promoter activity, The kIGFBP-4 promoter resid es within a CpG island and demonstrates strong basal activity in human osteoblast-like osteosarcoma and COS-7 monkey kidney cells. Transient transfection of cells with hIGFBP-4 promoter-linked deletion construc ts demonstrated that multiple cooperating cis-acting elements within 8 36 bp of the 5'-flanking region contributed to overall promoter streng th.