INTERACTION OF FLUORESCENT-PROBE 7-ANILINO-4-METHYLCOUMARIN-3-(P)BENZOIC ACID WITH EGG-ALBUMIN

The fluorescence properties of 7-anilino-4-methylcoumarin-3-(p)-benzoi c acid (AMCBA) synthesized as a fluorescent probe were measured in var ious solvents and in the egg albumin (EA) solution. The fluorescence o f AMCBA was significantly enhanced in the nonpolar solvents and in the egg albumin solution. The binding parameters of AMCBA to EA were esti mated fluorometrically. AMCBA which bound to EA was competitively disp laced by several beta-blockers and sulfonamide drugs. The binding para meters of beta-blockers and sulfonamides to EA were estimated by using a displacement method. A good correlation between binding constants o f sulfonamides or beta-blockers to EA and those partition coefficients was observed. These data suggest that AMCBA is one of the useful fluo rescent probes for studying the binding of drugs to polymeric pharmace utical additives, including EA.