ALTERNATIVE ALGORITHM FOR SIMULTANEOUS DETERMINATIONS OF COMPONENTS POORLY RESOLVED BY LIQUID-CHROMATOGRAPHY WITH MULTIWAVELENGTH DETECTION

This paper describes an alternative algorithm for a simultaneous multi component analysis with three-mode three-dimensional array data genera ted by liquid chromatography with diode array detection (LC-DAD). main ly based on a combination of two-step Moore-Penrose pseudoinverse comp utations based on a truncated singular value decomposition (TSVD) and one-step direct bilinear decomposition. It is modeled with a series of pure or mixed reference samples and then used to analyze several unkn own samples. The algorithm was evaluated by Monte-Carlo simulations of two synthetic LC-DAD data sets with different noise levels, and appli ed to the simultaneous determination of chlorobenzene and toluene by m eans of HPLC with a photodiode array detector. The obtained results sh ow that the proposed algorithm is suitable for simultaneous determinat ions of several components, of which the chromatographic and absorptio n heavily overlap each other, provided that their three-dimensional da ta have trilinear properties.