PRIMED IN-SITU LABELING (PRINS) AS A RATIONAL PROCEDURE FOR IDENTIFICATION OF MARKER CHROMOSOMES USING A PANEL OF PRIMERS DIFFERENTIALLY TAGGING THE HUMAN-CHROMOSOMES

Authors
HINDKJAER J BRANDT CA STROMKJAER H KOCH J KOLVRAA S BOLUND L
Citation
J. Hindkjaer et al., PRIMED IN-SITU LABELING (PRINS) AS A RATIONAL PROCEDURE FOR IDENTIFICATION OF MARKER CHROMOSOMES USING A PANEL OF PRIMERS DIFFERENTIALLY TAGGING THE HUMAN-CHROMOSOMES, Clinical genetics, 50(6), 1996, pp. 437-441
Citations number
8
Categorie Soggetti
Genetics & Heredity
Journal title
Clinical genetics → ACNP
ISSN journal
00099163
Volume
50
Issue
6
Year of publication
1996
Pages
437 - 441
Database
ISI
SICI code
0009-9163(1996)50:6<437:PIL(AA>2.0.ZU;2-F
Abstract
PRimed IN Situ labelling (PRINS) is a highly specific and sensitive te chnique for detecting DNA sequences on human chromosomes in situ. PRIN S is currently being introduced for research and routine analysis in c linical and cancer genetics. In this paper, we report a rational PRINS procedure for the rapid identification of marker chromosomes. Using t his method it is possible to test a sample from a patient with up to e ight different primers simultaneously on one slide. We have synthesize d oligonucleotide primers that can differentially tag the human chromo somes, and with the protocol presented in this report we are able to i dentify the chromosomal origin of a marker chromosome within 2 hours.