BIOCHEMICAL, MORPHOLOGICAL AND CYTOCHEMICAL STUDIES OF ENHANCED AUTOLYSIS OF SACCHAROMYCES-CEREVISIAE .1. BIOCHEMICAL-STUDIES

Biochemical aspects of induced autolysis of Saccharomyces cerevisisae were observed in the presence of various physical and chemical enhance rs of autolysis (increased temperature, changes of pH, NaCl, ethanol, fresh autolyzate). Direct assays of proteinases, nucleases, glucanases and acid phosphatases in homogenized autolyzed cells were performed. In addition, the degradation products of proteins, nucleic acids, poly saccharides and phosphate from phosphorylated compounds were determine d in the supernatant of autolyzate after centrifugation. These results suggested that the inducers affected transport processes and that the y had mostly negative effects on the activities of the above-mentioned enzymes.