BIOCHEMICAL, MORPHOLOGICAL AND CYTOCHEMICAL STUDIES OF ENHANCED AUTOLYSIS OF SACCHAROMYCES-CEREVISIAE .2. MORPHOLOGICAL AND CYTOCHEMICAL STUDIES

Morphological and cytochemical observation of Saccharomyces cerevisiae undergoing of induced autolysis were done in response to various chem ical inducers of autolysis (NaCl, ethanol, fresh autolyzate). Changes in the inner structure of yeast cells were monitored by transmission e lectron microscopy and the surface of the cell wall was observed by sc anning electron microscopy during autolysis. Cytochemical characteriza tion of autolyzed cells was performed using four synthetic substrates for determination of proteinase activities but only carboxypeptidase Y could be detected in the vacuolar membranes. The morphological studie s supported the data obtained from biochemical studies and confirmed t hat optimized conditions of autolysis have a significant effect on the structural changes of autolyzed yeast.