A SIMPLE METHOD FOR DEMONSTRATION OF EOSINOPHILS IN FROZEN-SECTIONS

Authors
FURGAL A LITWIN JA
Citation
A. Furgal et Ja. Litwin, A SIMPLE METHOD FOR DEMONSTRATION OF EOSINOPHILS IN FROZEN-SECTIONS, Folia histochemica et cytobiologica, 31(4), 1993, pp. 197-199
Citations number
15
Categorie Soggetti
Cytology & Histology
Journal title
Folia histochemica et cytobiologica → ACNP
ISSN journal
02398508
Volume
31
Issue
4
Year of publication
1993
Pages
197 - 199
Database
ISI
SICI code
0239-8508(1993)31:4<197:ASMFDO>2.0.ZU;2-S
Abstract
Tissue eosinophils can be easily and specifically demonstrated in froz en sections after substrateless incubation with 3,3'-diaminobenzidine (DAB) at neutral pH. Under such conditions, other cells possessing end ogenous peroxidatic activity (neutrophils, macrophages) do not stain, whereas staining of mitochondria-rich cells due to cytochrome oxidase activity can be avoided by a short prefixation of sections in glutaral dehyde-containing fixatives. The method can be useful in cases when a rapid screening of tissue eosinophils is required.