PRODUCTION OF THREONINE BY BREVIBACTERIUM-FLAVUM CONTAINING THREONINEBIOSYNTHESIS GENES FROM ESCHERICHIA-COLI

Authors
PATEK M HOCHMANNOVA J NESVERA J
Citation
M. Patek et al., PRODUCTION OF THREONINE BY BREVIBACTERIUM-FLAVUM CONTAINING THREONINEBIOSYNTHESIS GENES FROM ESCHERICHIA-COLI, Folia microbiologica, 38(5), 1993, pp. 355-359
Citations number
27
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Folia microbiologica → ACNP
ISSN journal
00155632
Volume
38
Issue
5
Year of publication
1993
Pages
355 - 359
Database
ISI
SICI code
0015-5632(1993)38:5<355:POTBBC>2.0.ZU;2-G
Abstract
Genes of the threonine operon of Escherichia coli were used for the co nstruction of a Brevibacterium flavum strain excreting threonine. Usin g the shuttle vector pCEM300 and a newly constructed shuttle vector pE C71 (7.1 kb, Km(r)/Nm(r)), various plasmids carrying E. coli thr genes were prepared. Mutants resistant to the threonine analog 2-amino-3-hy droxyvaleric acid (AHV) were isolated after the ethyl methanesulfonate treatment of B. flavum carrying these recombinant plasmids. A mutant B. flavum CCM 351 carrying the cloned genes thrA and thrB accumulated 12 g/L of threonine after 48 h of cultivation.