D1S80 POLYMORPHISM, INCLUDING A NEW VARIANT, IN A POPULATION-SAMPLE FROM BARCELONA (SPAIN) USING POLYMERASE CHAIN-REACTION

Allele and genotype frequencies for the D1S80 (MCT118) locus have been determined in a population sample from Barcelona (Spain) using the po lymerase chain reaction (PCR) amplification and nonradioactive detecti on. In a total of 216 unrelated individuals, 24 alleles (23 common and 1 rare variant) and 67 genotypes (64 common and 3 variants) were obse rved. The 216 individuals came from 162 blood samples taken for patern ity studies, 16 bloodstains from forensic cases, and 38 root hairs fro m normal individuals. The D1S80 locus demonstrated a heterozygosity of 0.7916, and a power of discrimination of 0.9731. The distribution of genotypes is in agreement with expected values according to the Hardy- Weinberg equilibrium. Additionally, the population from Barcelona diff ers, significantly, from the Finnish population and also, but with low er differences, from a U.S.A Caucasian population. (C) 1993 Wiley-Liss , Inc.