RAPID MAPPING OF COSMID CLONES ON PIG CHROMOSOMES BY FLUORESCENCE IN-SITU HYBRIDIZATION

Nineteen cosmids have been mapped to pig chromosomes by fluorescence i n situ hybridization. Two kinds of cosmid clones were isolated as pote ntial physical and genetic markers for the pig genome. Anonymous cosmi ds were obtained by screening a commercial cosmid library and were loc alized to Chromosomes (Chrs) 1, 2, 6, 7, 8, 10, 11, 12, 13, and 14. So me of these cosmids were found to reveal RFLP type DNA polymorphism. M icrosatellite-containing cosmid clones were isolated by screening a pi g cosmid library with a (CA)(10) probe and were regionally mapped to C hrs 2, 6, 7, 13, and 14. Ten of the 19 chromosomes in the pig were lab eled with these probes. Two-color fluorescence in situ hybridization w as used to increase the efficiency of the cosmid localizations.