KINETICS STUDY AND CHARACTERIZATION OF TARGET EXCRETED SECRETED ANTIGENS OF IMMUNOGLOBULIN-G, IMMUNOGLOBULIN-M, IMMUNOGLOBULIN-A, AND IMMUNOGLOBULIN-E ANTIBODIES FROM MICE INFECTED WITH DIFFERENT STRAINS OF TOXOPLASMA-GONDII

A kinetics study and characterisation of target excreted/secreted anti gens of immunoglobulin (Ig) G, M, A and E antibodies were realised by Western blotting with immune sera of mice inoculated with three strain s of Toxoplasma gondii: RH, C56 and S3. IgG antibodies of the immune s era recognised the major proteins of the three excreted/secreted antig en preparations with molecular masses of 30, 45, 63 and 77 kDa. IgM an tibodies recognised proteins revealed by IgG antibodies but with varia ble intensities; some proteins were revealed during a short period. Ig A antibodies did not recognise the 35-kDa antigen or the antigens infe rior to 28 kDa. The RH excreted/secreted antigens were revealed with t he highest intensity. The IgE antibodies were briefly detected in trac e amounts during period from the 20th to the 35th day. The RH strain w ith its excreted/secreted antigens had the best antigenicity and is a good model for immunoprotection studies.